Fig. 9
Lack of correlation between α-SNC secretion and JNK activation subsequent to ER stress or oxidative stress. a Differentiated PC12 cells expressing α-SNC or BDNF-differentiated SH-SY5Y cells expressing α-SNCA30P were treated overnight with tunicamycin (250 and 500 ng/ml) or thapsigargin (50 and 75 nM for PC12 cells, 25 and 50 nM for SHSY-5Y cells) or xanthine/xanthine oxidase (50 μM/0.02 U/ml and 100 μM/0.02 U/ml) before collection of conditioned medium and cell lysates for western blotting as indicated. b–e Bar graphs show integrated optical density (IOD) of western blot bands from above as indicated for antigen and cell line. Results are expressed as mean normalized IOD relative to control (no treatment) ±SEM and represent data from three independent experiments (*p < 0.05 by Student’s t test compared to “Control”)