Fig. 6

Administration of A-HO-1 protects neurons from SCI-induced neuronal death in vivo. a Expression of ATF4 and CHOP in injured spinal cords after SCI. Left panel: representative immunoblot image. Right panel: statistical analysis for the expression of ATF4 and CHOP. 0: immediately after SCI. N = 3 per group. *p < 0.05; ***p < 0.001 compared with “0” group. b Expression of HO-1, ATF4, and inflammasome components in injured spinal cords 24 h after SCI. Left panel: representative immunoblot image. Right panel: statistical analysis for the expression of each protein. Sham: sham control. N = 5 per group. **p < 0.01; ***p < 0.001 compared with sham control. ^# p < 0.05 compared with A-C group. c Expression of pro-caspase-1 and caspase-1 p20 in injured spinal cords 24 h after SCI. Left panel: representative immunoblot image. Right panel: statistical analysis for the expression of each protein. Sham: sham control. N = 5 per group. d Neuronal death was quantified by TUNEL 24 h after SCI. e BBB score after SCI. N = 10 per group. *p < 0.05; **p < 0.01; ***p < 0.001