Fig. 1

Brain PDGFRβ⁺ PCs express Iba1 following ischemia. At the ischemic site, PDGFRβ⁺ expression was specifically observed in PCs located near CD31⁺ endothelial cells (PDGFRβ (a, b green), CD31 (a, b red), DAPI (a, b blue)). On day 3 post-stroke, only a few PDGFRβ⁺ iPCs expressed Iba1 (c–f) (PDGFRβ (d, e red), Iba1 (d, f green), DAPI (d–f blue)) (arrow). However, some Iba1⁺PDGFRβ⁺ iPCs were observed in the ischemic areas on post-stroke day 5 (g–k) (PDGFRβ (h, i, k: red), Iba1 (h, j, k green), DAPI (h–k blue)) (arrows). The numbers of Iba1⁺ and PDGFRβ⁺ cells (l) and populations of Iba1⁺ cells relative to those of the PDGFRβ⁺ cells (m) localized to the ischemic core and peri-ischemic areas are shown. Scale bars = 100 μm (a), 50 μm (b), and 20 μm (d, h, k). Iba1 ionized calcium binding adaptor molecule 1, PDGFRβ platelet-derived growth factor receptor-β