Fig. 5

Microglial cells from CP brains showed lower migration velocity and less persistent distance. a Microglial migration velocity distribution for healthy and CP brain slices. Microglia from healthy brain slices showed higher average migration velocity during the observation period, especially at high migration velocity range, than the microglia from CP brain slices. b Persistent distance of microglial migration trajectory is greater in the healthy rabbit brain slices. Persistent migration was defined as the length (>10 μm) traveled by a microglial cell before it makes a significant change in direction (absolute angle between previous direction and new direction <70°) *p < 0.05 For microglia from healthy brain slice, population median 30.3 μm, 20 % percentile, 16.6 μm, 80 % percentile, 44.4 μm; for microglia from CP brain slice, population median 15.22 μm, 20 % percentile, 11.6 μm, 80 % percentile, 17.9 μm