Fig. 3

IL-2Ra (CD25) and IL-7Ra (CD127) expression on CD3+CD4+ lymph node cells. Cells were gated for CD3+CD4+Foxp3- (i.e., Th) and CD3+CD4+Foxp3+ (i.e., Treg) as in Fig. 2. Representative histograms showing the level of expression of IL-2Ra (a) and IL-7Ra (d) in Th (dark gray) and Treg (light gray) T cell populations. Bar graphs showing the average percentage + SD of cells expressing CD25 (b, c) or CD127 (e, f). b, e CD3+CD4+Foxp3- cells. c, f CD3+CD4+Foxp3+ cells. g Representative dot plot of CD3+CD4+Foxp3+ cells expressing CD127 and CCR6. h Bar graphs showing the distribution of CD3+CD4+Foxp3+ cells (average percentage + SD) according to their expression of CD127 and CCR6. One-way ANOVA followed by Tukey HSD. Asterisk means different from naive; A means different from adjuvant; dagger symbol means different from naive and adjuvant; closed circle means different from high-dose α-syn and Nα-syn; open circle means different from the other α-syn variant independent of dose; not equal to symbol means different from all; double dagger symbol means different from the other α-syn variant at a different dose; plus-minus sign means different from the same α-syn variant at different dose. p < 0.05. N = 8–10, divided into three to five independent experiments