Fig. 2

CNS-infiltrating Th17 cells are significantly elevated in CXCR3^−/− mice compared with WT mice. Mononuclear cells were isolated from the spinal cords of MOG-immunized WT (n = 35) and CXCR3^−/− (n = 34) mice at the peak of disease. Total and subpopulations of CNS-infiltrating lymphocytes were determined by surface staining and intracellular staining using flow cytometry as described in the “Methods” section. a The gating strategy is shown: a mononuclear cells from spinal cords were gated; b the live mononuclear cells were further gated from total mononuclear cells shown in a; c CD4⁺ T cells were further gated from live mononuclear cells shown in b; and d the subpopulation of CD4⁺IFN-γ⁺ T cells and CD4⁺IL-17⁺ T cells were identified from CD4⁺ T cells shown in c. b Total CNS-infiltrating leukocytes. c CNS-infiltrating CD4⁺ T cells. d CNS-infiltrating CD4⁺IFN-γ⁺ T cells. e CNS-infiltrating CD4⁺IL-17⁺ T cells. Each mouse is represented by a symbol. Data shown are from six independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001