Table 3 Primary antibodies and their dilutions
From: Complement activation at the motor end-plates in amyotrophic lateral sclerosis
Antigen | Species | Specificity | Type | Dilution | Art.#/company |
|---|---|---|---|---|---|
Neurofilament heavy chain (NF-H) | Rabbit polyclonal | Anti-neurofilament | Anti-human | 1:1000 | ab8135/Abcam |
MAC (ae11 clone) | Mouse monoclonal | Anti-C5b-9 complex | Anti-human | 1:200 | M0777/Dako |
FITC-conjugated C1q complement | Polyclonal rabbit | Anti-C1q | Anti-human | 1:50 | F0254/Dako |
FITC-conjugated DAF | Mouse monoclonal | Anti-CD55 | Anti-human | 1:40 | 555693/BD Pharmigen |
CD59 | Mouse monoclonal | Anti-CD59 | Anti-human | 1:50 | HM2120/Hycult |
C1q | Polyclonal rabbit | Anti-C1q | Anti-human | 1:50 | A0136/Dako |
FITC-conjugated C3c complement | Polyclonal rabbit | Anti-C3 | Anti-human | 1:50 | ab4212/Abcam |
DAF | Mouse monoclonal | Anti-CD55 | Anti-human | 1:150 | Ab20145/Abcam |
Synaptophysin | Rabbit monoclonal | Anti-synaptophysin | Anti-human | 1:50 | RM-9111-S/Thermo Scientific |
Synaptophysin | Mouse monoclonal | Anti-synaptophysin | Anti-human | 1:200 | M0776/Dako |
S100b | Rabbit polyclonal | Anti-S100b | Anti-human | 1:100 | Z0311/Dako |
S100b | Mouse monoclonal | Anti-S100b | Anti-human | 1:500 | S2532/Sigma |