Fig. 7

NLRP3 KO mice had reduced caspase-1 activation and IL-1β production in the diencephalon. The samples came from two mice per experiment, and the results are representative of three independent experiments (a). Cas-p10 induction (b) and IL-1β production (c) were quantified by measuring band intensities using ImageJ software. The values were normalized to β-actin. (d) DAPI (blue), Caspase-1 (green) and IL-1β (red) immunofluorescent staining in diencephalon sections; scale bar, 20 μm. The merged image shows the co-localisation of caspase-1 and IL-1β protein. **p < 0.01, *p < 0.05. Mean ± SEM of three samples