Fig. 1

Oxytocin receptor (OTR) expression in BV-2 cells and primary microglia. a Immunostaining with anti-OTR (red) in BV-2 cells and primary microglia. Nuclei were counterstained with DAPI (blue). Scale bar = 50 μm. b OTR mRNA expression in BV-2 cells after LPS challenged were analyzed by RT-PCR, β-actin was used to evaluate loading. c OTR protein expression in BV-2 cells after LPS challenged was analyzed by Western blotting, and β-actin was used to evaluate protein loading. d OTR mRNA expression in primary microglia after LPS challenged were analyzed by RT-PCR, β-actin was used to evaluate loading. e OTR protein expression in primary microglia after LPS challenged was analyzed by Western blotting, and β-actin was used to evaluate protein loading