Fig. 3
IDO ablation results in early and increased CNS infiltration by myeloid and lymphoid leukocytes. a, b The frequency and number of Ly-6Chi monocytes and Ly-6Ghi granulocytes in the CNS. The frequency (a) and total number (b) of Ly-6Chi monocytes and Ly-6Ghi granulocytes in the CNS were determined by flow cytometric analyses 3 and 5 dpi using vigorous heart perfusion. Values presented in the representative dot plots denote the average percentage of the indicated population after gating on CD11b+ cells (n = 4–5). c, d Accumulated number of NK cells, CD4+, and CD8+ T-cells in the CNS. Total accumulated number of NK cells (CD3−NK1.1+DX5+), CD4+ (CD3+CD4+), and CD8+ (CD3+CD8+) T-cells in the CNS were enumerated by flow cytometric analysis 3 and 5 dpi. e The expression of TNF-α and CC chemokines in the CNS. The expression levels of TNF-α and CC chemokines were determined by real-time qRT-PCR using total RNA extracted from brain tissue 2 dpi. Data show the average ± SD of the indicated cell populations derived from at least five mice per group. *p < 0.05; **p < 0.01; ***p < 0.001 compared with levels in the indicated groups