Fig. 7

IFN-I innate immune responses of IDO-ablated myeloid-derived cells after JEV infection. Primary bone marrow-derived conventional DCs (BMDCs), plasmacytoid DCs (pDCs), and macrophages (BMDMs) recovered from BL/6 and IDO KO mice were infected with JEV at MOIs of 0.1, 1.0, and 10 for viral replication and 10 for cytokine expression. JEV replication and the expression of cytokines and IFN-α/β were evaluated by real-time qRT-PCR using extracted total RNA. a–c JEV replication in BMDCs, BMDMs, and pDCs. d, e IFN-α/β, IL-6, and TNF-α expression in BMDCs, pDCs, and BMDMs. The bar charts show the average ± SD of the values derived from BMDCs, BMDMs, and pDCs assayed in quadruplicates. *p < 0.05; **p < 0.01; p < 0.001 compared with levels in the indicated groups