Table 1 Specific primers for cytokines, chemokines, type I IFNs, and ISGs used in real-time qRT-PCR
| Gene name | Primer sequence (5′-3′) | Position cDNA | GeneBank ID |
|---|---|---|---|
| IL-6 |
FP: TGG GAA ATC GTG GAA ATG AG RP: CTC TGA AGG ACT CTG GCT TTG |
209–228 442–462 | NM_031168 |
| TNF-α |
FP: CGT CGT AGC AAA CCA CCA AG RP: TTG AAG AGA ACC TGG GAG TAG ACA |
438–457 564–587 | NM_013693 |
| IFN-α |
FP: TGTCTGATGCAGCAGGTGG RP: AAGACAGGGCTCTCCAGAC |
367–385 514–532 | NM_008334.3 |
| IFN-β |
FP: TCCAAGAAAGGACGAACATTCG RP: TGAGGACATCTCCCACGTCAA |
106–121 399–419 | NM_010510 |
| CCL2 |
FP: AAA AAC CTG GAT CGG AAC CAA RP: CGG GTC AAC TTC ACA TTC AAA G |
347–367 426–447 | NM_011333 |
| CCL3 |
FP: CCA AGT CTT CTC AGC GCC AT RP: GAA TCT TCC GGC TGT AGG AGA AG |
158–177 206–228 | NM_011337.2 |
| CCL4 |
FP: TTC TGT GCT CCA GGG TTC TC RP: GAG GAG GCC TCT CCT GAA GT |
128–147 388–407 | NM_013652.2 |
| CCL5 |
FP: CCC TCA CCA TCA TCC TCA CT RP: CTT CTT CTC TGG GTT GGC AC |
77–96 275–294 | NM_013653.3 |
| IRF3 |
FP: GAT GGA GAG GTC CAC AAG GA RP: GAG TGT AGC GTG GGG AGT GT |
1170–1189 1259–1278 | NM_016849 |
| IRF5 |
FP: GGA AGA AAT GAA GCC AGC AG RP: ACC CTG GGG TAA TTG GAC TC |
1931–1950 2001–2020 | NM_001252382.1 |
| IRF7 |
FP: CCT CTT GCT TCA GGT TCT GC RP: GCT GCA TAG GGT TCC TCG TA |
980–999 1080–1099 | NM_016850.3 |
| RIG-I |
FP: CCA CCT ACA TCC TCA GCT ACA TGA RP: TGG GCC CTT GTT GTT CTT CT |
194–217 260–279 | NM_172689 |
| MDA5 |
FP: GGC ACC ATG GGA AGT GAT T RP: ATT TGG TAA GGC CTG AGC TG |
1178–1196 1247–1266 | NM_027835 |
| PKR |
FP: AAC TTC TTC ACA CGT GCT TC RP: CAT TCA GCC AAG GTC TTC AG |
1517–1536 1678–1697 | NM_011163 |
| STAT1 |
FP: AAG CGA ACT GGA TAC ATC A RP: CCG GGA CAT CTC ATC AAA C |
2093–2111 2194–2212 | U06924.1 |
| 2′5′-Oas1 |
FP: CCA TCC TCA AGT GGA CAA GAA CTG RP: TTG GGC TTT GGG CAC CTT C |
1235–1258 1359–1377 | AF466822.1 |
| Oasl-1 |
FP: CCA GGA AGA AGC CAA GCA CCA TC RP: AGG TTA CTG AGC CCA AGG TCC ATC |
377–399 449–472 | NM_145209.3 |
| Mx1 |
FP: CAG CAC CTG ATG GCC TAT CA RP: ACG TCT GGA GCA TGA AGA ACT G |
2276–2295 2363–2342 | NM_010846.1 |
| Mx2 |
FP: AGG CTC ACA ACC GCA TCT RP: GCT CAG CAA ACA TTT TCA GG |
1858–1875 1910–1929 | NM_013606.1 |
| ISG49 |
FP: GCC GTT ACA GGG AAA TAC TGG RP: CCT CAA CAT CGG GGC TCT |
919–939 1126–1143 | NM_010501.2 |
| ISG54 |
FP: GGG AAA GCA GAG GAA ATC AA RP: TGA AAG TTG CCA TAC AGA AG |
1918–1937 2005–2024 | NM_008332.3 |
| ISG56 |
FP: CAG AAG CAC ACA TTG AAG AA RP: TGT AAG TAG CCA GAG GAA GG |
774–793 911–930 | NM_008331.3 |
| β-actin |
FP: TGG AAT CCC TGT GGG ACC ATG AAA C RP: TAA AAC GCA GCT CAG TAA CAG TCC G |
885–909 1209–1233 | NM_007393.3 |