Fig. 6

Aldara treatment induces the infiltration of CD3⁺ cells into the brain. Mice were treated with 80-mg Aldara cream or control cream every 24 h for one, three or five consecutive days. Mice were euthanised 24 h following the final application. Perfused brains were fixed in formalin and embedded in paraffin before being sectioned to 7 μm. a Three sections from each brain were stained for CD3. Representative sections of the hippocampus and cerebellum are shown at ×400 magnification or ×100 magnification (□). Arrows indicate CD3⁺ cells. b Whole brain CD3⁺ infiltrates were counted blind. n = 4 mice per group. Significance was determined using two-way ANOVA with Bonferroni multiple comparison post-tests ***p ≤ 0.001