Fig. 2

IFNγ preserves the NSPC population, but not early neurons. CD46+ (a–d) and CD46+/IFNγ-KO (e–h) control and MV-infected neonatal whole-brain isolates labeled for multipotent NSPCs (nestin: a, e) and cells specified to the neuronal lineage (CD24: b, f; DCX: c, g; NeuN: d, h), at 3, 7, and 10 dpi. The percentage of nestin + cells was significantly reduced at 7 and 10 dpi in MV-infected IFNγ-KO (e), but not MV-infected CD46+ mice (a). Significant CD24+ (b, f) and DCX+ (c, g) immature neuron cell loss occurred in both genotypes. Mature NeuN+ cell number was unaffected (d, h). *p < 0.01 significantly different uninfected versus MV-infected; #p < 0.05 significantly different between dpi