Fig. 3

IFNγ does not alter glial differentiation. CD46+ (a–d) and CD46+/IFNγ-KO (e-h) control and MV-infected neonatal whole-brain isolates were labeled for early glial precursors (A2B5: a, e), oligodendrocytes (O4: b, f) and astrocytes (GFAP: c, g). The average GFAP mean fluorescent intensity (MFI) was quantified and normalized to the MFI of the isotype control (d, h). There was no significant difference between control and MV-infected pups for the glial cell populations in either genotype. A significant decrease in %A2B5+ cells was observed in CD46+ (a), but not IFNγ-KO mice (d) mice from 3 to 10 dpi and a significant increase in O4+ oligodendrocytes was observed from 3 to 10 dpi in both genotypes (b, e). Age-dependent differences in GFAP expression also occurred (d, h). *p < 0.01 significantly different uninfected versus MV-infected; #p < 0.05