Fig. 5
From: Clozapine metabolites protect dopaminergic neurons through inhibition of microglial NADPH oxidase
Gp91phox genetic ablation blocks the anti-inflammatory and neuroprotective effects of CNO and NDC. Neuron-glia cultures prepared from wild-type (gp91phox+/+ ) and NOX2-deficient (gp9 phox−/−) mice were pretreated with vehicle or CNO (0.01 μM) or NDC (0.1 μM) for 30 min followed by LPS (20 ng/ml for gp91phox+/+or 50 ng/ml for gp9phox−/−) stimulation. a, b The supernatant levels of TNF-α were detected 3 h after LPS treatment. c, d The protective effects of CNO and NDC against LPS-induced dopaminergic damage were assessed by DA uptake assay 7 days after treatment. Results are expressed as the mean ± SEM or as a percentage of vehicle controls (mean ± SEM) from three independent experiments performed in triplicate. **p < 0.01, ## p < 0.01. NS not significant