Fig. 3
From: Complement system activation contributes to the ependymal damage induced by microbial neuraminidase
Western blot detection of C1q and MBL in ependymal wall extracts. Protein extracts were prepared from the lateral wall of rats injected with NA (R4–R6) and non-injected control rats (R1-R3). In order to increase the amount of target proteins (C1q and MBL) loaded in the gel, they were immunoprecipitated from the extracts prior to the Western blot. Neither C1q (a) nor MBL (b) were detected in ependymal wall extracts, regardless of the treatment with NA. Positive controls (C+) consisted in rat serum samples are subjected to the same procedure; the specificity of the antibodies was checked by omitting the primary antibodies (1st Ab bypass)