Fig. 4
Representative images of tibialis cranialis muscle in the horizontal plane showing a greater number of neuromuscular junctions in mutant mice. Neuromuscular junctions immunostained with anti-α-bungarotoxin tetramethylrhodamine conjugate (red). Muscle myofibrils (blue) by using optical system second harmonic generation (SHC). (a–b) Contralateral and (c–d) ipsilateral sides of tibialis cranialis muscle, respectively, in C3H/HePas and C3H/HeJ mice. (e–f) Graphs of the number of neuromuscular junctions per muscle area. (g–h) Neuromuscular junctions immunostained in details. Note that the functional recovery observed in mutant mice might be a result of an increase of nerve terminal sprouts. Scale bar: 100 μm