Fig. 3

Immunocytochemical and confocal analysis of the effects on FK506-binding proteins in gp120 tg mice. Non-tg and gp120 tg mice were treated with vehicle or FK506 for 4 weeks and immunostained with antibodies against FKBP12 and 51. Representative images are shown from the fronto-temporal cortex. a Photomicrographs and b image analysis of FKBP12-positive neurons showing that FK506 treatment increased FKBP12 in the non-tg and gp120 tg mice. c Photomicrographs and d image analysis of FKBP51-positive neurons showing that FK506 treatment increased FKBP51 in the non-tg and gp120 tg mice. e Confocal images showing colocalization of FKBP12 (red, upper panel) and FKBP51 (red, lower panel), respectively, with dendritic marker MAP2 (green). Scale bar in a = 10 μm. Scale bar in e = 10 μm. *p value <0.05 by one-way ANOVA and post hoc Dunnett’s compared to vehicle; ^# p value <0.05 by one-way ANOVA and Tukey-Krammer post hoc test compared to vehicle-treated gp120 mice; N = 10; age = 12 months of age