Fig. 8

Calcium dynamics by live imaging in the barrel cortex neurons in gp120 tg mice treated with FK506. a Composite two-photon image of the calcium indicator dye, OGB (green), and astrocyte marker, SR101 (yellow-orange). Representative two-photon images indicating the SR101-positive structures in each mouse type. Regions of interest for each image were extracted; neurons are indicated in green and astrocytes are in red. b The time course for the calcium signal was extracted based on regions of interest identified in the previous panel for 1 Hz. The arrows indicate the onset of the stimulus. c Visual representation of average calcium peak shapes for non-tg, GP120, and GP120 mice treated with FK506 mice using 1Hz stimulus frequency at a depth of 150 μm. d There was no significant difference between normalized calcium traces from barrel cortex neurons that were quantified using area under the curve (AUC). Scale bar for photomicrographs is 10 μm