Fig. 5
From: Endogenous analgesia mediated by CD4+ T lymphocytes is dependent on enkephalins in mice
Anti-β-endorphin polyclonal IgG antibodies do not bind to activated lymphocytes from pre-proenkephalin knockout mice as assessed by cytofluorometry. CD4+ T lymphocytes isolated from wild-type C57Bl/6 (PENK+/+) mice (left panels) or pre-proenkephalin knockout (PENK−/−) mice (right panels) were stimulated with both anti-CD3 and anti-CD28 mAbs for 6 days and intracellularly stained with either control rabbit non-immune serum IgG (upper panels), rabbit anti-β-endorphin polyclonal IgG antibodies (middle panels), or rabbit anti-Met-enkephalin polyclonal IgG antibodies (lower panels). The figure shows one representative experiment out of three performed