Fig. 4

IL4 gene therapy induces M2 markers and IL6 expression in CNS-infiltrating CD11b⁺ cells in vivo. mRNA levels of IL6, IL-1β, and YM1 were measured by real-time RT-PCR in CD11b⁺ cells recovered from the CNS of EAE mice treated intracisternally with an IL4-expressing (IL4) or a GFP-expressing (GFP) lentivirus. IL4 gene therapy induces a decrease of IL-1β (a), and an increase of IL6 (b) and Ym1 (c), as compared to control-treated mice. n = 3 for each group. Gapdh has been used as a housekeeping gene. Data are shown as arbitrary units (AU ± standard deviation). *P < 0.05 (t test)