Fig. 1

Model of TLR4 activation of rat microglia in vivo. a Immunofluorescence images of adjacent 10-μm coronal brain sections 6 h after intrastriatal infusion of LPS (rate, 5 μg/day); Iba1⁺ cells (green, upper) versus P2Y12⁺ cells (green, lower); dotted line demarcates the pial layer separating the subarachnoid space from the parenchyma; nuclei labeled with DAPI (blue); scale bar 40 μm. b Grayscale images of brain sections of control (CTR) versus 24 h after intrastriatal infusion of LPS (LPS 24 h); cc corpus callosum; P2Y12 labeling (black); morphological changes in activated microglia are shown in the inset; scale bar 40 μm. c High magnification immunofluorescence images representative of microglia under control (CTR) conditions versus 24 h after intrastriatal infusion of LPS (LPS 24 h); P2Y12 (green) and ED1 (red); nuclei labeled with DAPI (blue)