Fig. 9

Sur1-Trpm4 regulates induction of NOS2 protein in murine N9 microglia. a, b Immunoblot (a) and densitometric analysis (b) for NOS2 protein in N9 microglia under control (CTR) conditions or following 24-h exposure to LPS alone (1 μg/mL), LPS plus glibenclamide (Glib; 30 μM), or LPS plus 9-phenanthrol (9Phe; 5 μM); quantitative data were normalized to a loading control for total protein, HSC70, and to values with LPS alone; five replicates; **p < 0.01. c Percent nitrite in the medium of N9 microglial cultures under control (CTR) conditions or following 24-h exposure to LPS alone (1 μg/mL), LPS plus glibenclamide (Glib; 30 μM), or LPS plus 9-phenanthrol (9Phe; 5 μM); nitrite was measured using the Griess assay; data normalized to values with LPS alone, with maximum nitrite ranging from 8 to 13 μM per experiment; three replicates. d Concentration-response relationship for LPS-induced nitrite in the medium versus glibenclamide; EC₅₀, 24 μM; experiments performed in 5 % fetal bovine serum; three replicates