Fig. 3

Combined ex vivo autoradiography and IBA-1 immunohistochemistry show increased [¹⁸F]DPA-714 uptake in microglia after closed head weight-drop TBI in mice. a Representative images of [¹⁸F]DPA-714 autoradiography and IBA-1 immunohistochemical staining on the same section of a sham, mTBI, and TBI mouse on day 7. The middle panel show merged images in each case. Red asterisks on the low magnification images denote areas depicted in higher magnification below each image. Red arrows mark IBA-1 positive cells, scale bar = 50 μm. b Box-plots representing calculated TBI/N ratios of [¹⁸F]DPA-714 binding in sham operated (n = 5), mTBI (n = 11), and TBI (n = 6) mice on day 7 and in c on day 16 in three mTBI and three TBI mice. d IBA-1 positive cell counts on day 7 were derived from the sections used for calculation of the TBI/N ratios in b in sham (n = 5), mTBI (n = 11), and TBI (n = 6) mice. e show IBA-1 counts on day 16 in two mTBI and three TBI mice used for TBI/N ratio in c. ***p < 0.001, **p < 0.01, *p < 0.05