Table 3 Overview of used antibodies
From: Expression of IL-1β in rhesus EAE and MS lesions is mainly induced in the CNS itself
Source | Species | Dilution | |
|---|---|---|---|
Primary antibodies | |||
IL-1β (clone C-20) | Santa Cruz Biotechnology | Goat | Frozen: 1:100 |
Parafin: 1:250 | |||
PLP (clone plpc1) | AbD Serotec | Mouse | Frozen: 1:500 |
Parafin: 1:300 | |||
HLA-DR (clone LN3) | eBioscience | Mouse | Frozen: 1:750 |
Parafin: 1:500 | |||
Iba-1 | Wako | Rabbit | 1:250 |
MRP14 (clone S36.48) | BMA Biomedicals | Mouse | 1:100 |
CCL22 | Abcam | Rabbit | 1:100 |
CD200R (clone OX108) | AbD Serotec | Mouse | 1:50 |
CD40 (clone LOB7/6) | AbD Serotec | Mouse | 1:50 |
CD74 (clone By2) | Santa Cruz Biotechnology | Mouse | 1:1600 |
MR (clone 19.2) | BD Pharmingen | Mouse | 1:150 |
Isotype control | Southern Biotech | Goat | 1:2500 |
Secondary antibodies | |||
EnVision HRP-labeled anti-mouse/rabbit polymer | DAKO | – | undiluted |
Biotinylated anti-sheep/goat | Amersham | Donkey | 1:200 |
Avidin-HRP | Sigma Aldrich | – | 1:100 |
Anti-goat HRP | Jackson ImmunoResearch | Donkey | 1:100 |
Anti-mouse IgG alkaline phosphatase | DAKO | Goat | 1:250 |
Anti-rabbit IgG alkaline phosphatase | Southern Biotech | Goat | 1:250 |
Avidin-CY2 | Jackson ImmunoResearch | – | 1:150 |
Anti-rabbit TRITC | Jackson ImmunoResearch | Donkey | 1:100 |
Anti-mouse TRITC | Jackson ImmunoResearch | Donkey | 1:50 |