Fig. 9
From: Augmented expression of TSPO after intracerebral hemorrhage: a role in inflammation?
Ro5-4864-attenuated hemin-induced release of TNF-α and IL-6. RAW 264.7 cells were plated overnight and TSPO agonist, Ro5-4864, was added 1 h prior to hemin (30 μM) addition and the hemin treatment was conducted in the presence of Ro5-4864 and the supernatant was collected. The release of TNF-α (a) and IL-6 (b) into the supernatant was estimated using ELISA as per manufacturer’s instructions. (RayBiotech, Inc., Norcross, GA, USA). Ro5-4864 treatment significantly attenuated the hemin-induced release of TNF-α and IL-6. n = 4–9; *p < 0.05, ***p < 0.001 vs. hemin alone