Fig. 5

Phenotypic profile of CNS infiltrating cells. BALB/c and ST2^−/− mice were infected with 10⁴ PFU of ROCV. CNS infiltrating cells were collected 9 days post-infection, and stimulated for 4 h with phorbol-12-myristate-13-acetate (PMA) and ionomycin in the presence of brefeldin A. The frequency of CD3⁺ and CD4⁺ or CD8⁺ T cells (a, e) and IL-10 (b, f), IFN-γ (c, g), and TNF-α (d, h)-producing cells was analyzed by flow cytometry (FACS). *p < 0.05, **p < 0.001. NS non-significant. Data are representative as the mean of three independent experiments with at least five animals per group. Statistical analysis was performed by Student t test using the software GraphPad Prism