Fig. 4

CCL20 neutralization reduces leukocyte infiltration. a Immunostaining of the spinal cord sections at 7 days post-SCI shows various amounts of CD45-positive cells in the different groups. CD45-positive cells are stained green, while the nuclei are stained blue. The immunostained areas in the white box are magnified below. Scale bars are 50 and 100 μm, respectively. b The percentage of CD45-positive cells indicates that leukocyte infiltration, as well as neuroinflammation during secondary injury, was significantly alleviated by neutralizing CCL20. ⁺ P < 0.05, compared with the sham group; ^* P < 0.05, compared with the CCL20 mAb group