Fig. 3

Expression of Gas1 in primary astrocytes and influence of LPS on cell viability of astrocytes. a Immunoblots of Gas1 expression and cleaved caspase-3 in the astrocytes with increasing culture times of 24, 48, 72, and 96 h. b Immunoblots of Gas1 expression levels in the astrocytes with increasing cell density from the cerebral cortex and substantia nigra. c Cell viability of the astrocytes with LPS insult at increasing concentrations of 0.1, 0.5, 1, 5, 10, and 20 μg/ml. d Cell viability of the astrocytes under control and LPS (1 μg/ml) insult with increasing times of 24, 48, 72, and 96 h