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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Gas1 up-regulation is inducible and contributes to cell apoptosis in reactive astrocytes in the substantia nigra of LPS and MPTP models

Fig. 6

Effect of siRNA knockdown of Gas1 expression on survival, apoptosis, and proliferation of the primary astrocytes. a siRNA knockdown efficiency of three small interfering RNA specific towards Gas1 (siGas1) determined by real time qPCR. *P < 0.05, ***P < 0.001 vs control (mean ± S.E.M., n = 3). b Effective working time of siGas1 measured by immunoblot. c, d Influence of down-regulated Gas1 on cell viability or necrotic death of astrocytes of control and treated with LPS, IFNγ and TNFα by CCK-8 and LDH assay. ANOVA, *P < 0.05, **P < 0.01, ***P < 0.001 vs control, n.s. no significance (mean ± S.E.M., n = 3). e Influence of down-regulated Gas1 on survival, apoptosis, and necrosis of primary astrocytes by flow cytometry. f Influence of Gas1 siRNA on apoptosis of cultured astrocytes treated with LPS, IFNγ, and TNFα by TUNEL staining. g Influence of Gas1 siRNA on proliferation state of astrocytes treated with LPS, IFNγ, and TNFα by Ki-67/BrdU double labeling

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Journal of Neuroinflammation

ISSN: 1742-2094