Fig. 1
Clinical progression of EAE in anti-hnRNP A1-M9-injected mice compared to controls. a EAE was induced in C57BL/6J mice with active immunization using MOG35–55. At the first clinical signs of disease (limp tail) (designated as day 0 in this graph), the mice were injected with 100 μg of PBS (black), isotype-matched IgG2b (green), or anti-hnRNP A1-M9 (red) antibodies. Injections were given at days 0, 2, and 4 for a total of three injections (arrows). EAE mice were scored daily from the disease onset using the following standard scale: 1—limp tail; 2—hind limb weakness/wobbly gait; 3—hind limb paralysis; 4—hind limb paralysis/weakness of front limbs; and 5—moribund. Quantification was performed in GraphPad Prism software. Statistical significance was analyzed by two-way repeated measures analysis of variance (ANOVA) (p ≤ 0.05). b Anti-hnRNP A1-M9 antibody-treated mice developed increased spastic paralysis compared to an isotype control IgG2b and PBS-injected animals, which develop flaccid paralysis