Fig. 2

The number of Gal-3+ cells in the SVZ decreases after cuprizone treatment. a, c Representative images of Gal-3+ cells in the SVZ and CC after cuprizone treatment. Scale bar 100 μm. White dotted lines define SVZ boundaries. b, d Graphs demonstrating the density of Gal-3+ cells in the SVZ (b) and CC (d). Statistics calculated as one-way ANOVA with N = 4. Graphs show mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. e Representative images of the SVZ from 6-week cuprizone or remyelination brains showing Gal-3 (green) colocalization with GFAP+ astrocytes/neural stem cells, Mash-1+ TAPs and CD45+ hematopoietic cells (which includes CD45+/Iba1+ microglia), and lack of Gal-3 colocalization with Dcx+ neuroblasts and Olig2+ oligodendrocytes. We used N = 4 for all groups. Dotted box shows magnified region. Scale bars 50 μm. f–h Graphs showing the quantification of colocalization in the CC. Statistics calculated as one-way ANOVA with N = 4 for f and g. No statistics performed for h. Graphs show mean ± SEM. ***p < 0.001. Note that GFAP+ and CD45+ cells were quantified in different experiments, so aggregates in h will not necessarily equal 100 %