Fig. 5

SVZ proliferation decreases after cuprizone treatment. a High-magnification images of the dorsolateral SVZ comparing PHi3+ cells in Gal-3 ^+/+ and Gal-3 ^−/− mice across treatment groups. Scale bar 100 μm. b, c Quantification of PHi3+ cells in the SVZ (b) and CC (c). d–g Example of Ki67 and Mash1 double immunohistochemistry in the SVZ of a WT mouse in the remyelination group. Arrow points to a double-labeled cell. Scale bar 50 μm. h, i Quantification of Ki67+ and Mash1+/Ki67+ cells in the SVZ. Statistics used were one-way ANOVA with Tukey’s post hoc test. Graphs show mean ± SEM. *p < 0.05, **p < 0.01. j, k Il-1β immunohistochemistry in controls and the 6-week cuprizone group. Note that Il-1β was barely detectable in controls but upregulated in the CC and SVZ in WT and Gal-3 ^−/− mice in the 6-week cuprizone group. Scale bar 50 μm