Fig. 5

CXCR5 is essential for pIONL-induced mechanical allodynia. a Mechanical sensitivity assessed by Von Frey test was indistinguishable in WT and KO mice. b pIONL-induced mechanical allodynia was significantly reduced in Cxcr5 KO mice, compared to WT mice. *P < 0.05, **P < 0.01. Two-way repeated measures ANOVA followed by Bonferroni test. c Pretreatment with LV-Cxcr5 shRNA increased the contact time between 3 and 21 days after the operation, compared to LV-NC-injected mice. *P < 0.05, ***P < 0.001. Two-way repeated measures ANOVA followed by Bonferroni test. d Posttreatment with LV-Cxcr5 shRNA increased the contact time between 7 and 21 days after the operation, compared to LV-NC treatment. *P < 0.05, **P < 0.01, ***P < 0.001. Two-way repeated measures ANOVA followed by Bonferroni test. e Real-time PCR assay of Cxcr5 shows that pretreatment with LV-Cxcr5 shRNA inhibited pIONL-induced Cxcr5 upregulation. *P < 0.05, **P < 0.01, ***P < 0.001. Student’s t test