Fig. 8

Intra-TG injection of CXCL13 induces CXCR5/ERK-dependent mechanical allodynia and proinflammatory cytokines production. a, b Intra-TG injection of CXCL13 (100 ng) induced mechanical allodynia in WT mice (a), but not in Cxcr5 KO mice (b). ***P < 0.001, WT-vehicle vs. WT-CXL13. Two-way repeated measures ANOVA followed by Bonferroni test. c Western blot showing that intra-TG CXCL13 significantly increased pERK expression in the TG in WT mice, but not in Cxcr5 KO mice. *P < 0.05. Student’s t test. d Intra-TG injection of CXCL13 increases TNF-α and IL-1β expression at 1 and 3 h in WT mice, but not in Cxcr5 KO mice. *P < 0.05, **P < 0.01. One-way ANOVA followed by Bonferroni test. e Intra-TG injection of PD98059, Etanercept, or Diacerein partially blocked intra-TG CXCL13-induced mechanical allodynia. *P < 0.05, **P < 0.01, ***P < 0.001. Two-way repeated measures ANOVA followed by Bonferroni test