Fig. 1
CCR5 is essential for protection against JE but dispensable for control of JEV replication. a Susceptibility of CCR5-ablated mice to JE. Ccr5+/+ and Ccr5−/− mice (4 to 6 weeks old, n = 10–15) was inoculated i.p. with JEV (3.0 × 107 pfu). The survival rate was examined over 15 days. b Ratio of mice showing neurological disorders during JE progression. Mice infected with JEV were examined every 6 h from 4 to 8 dpi. c Changes in body weight. Data are average percentages ± SD of body weight relative to that at the time of challenge. d Viral burden in lymphoid and inflammatory tissues during JE. Viral burden in lymphoid (spleen) and inflammatory tissues (brain and spinal cord) of infected mice (n = 5–6) were assessed by real-time qRT-PCR at the indicated time points. Viral RNA load was expressed as viral RNA copy number per microgram of total RNA (n = 5–7). *p < 0.05; **p < 0.01 compared to the levels in the corresponding groups