Fig. 1

ZSTK474 alleviates neurological deficits and reduces infarct volume in a model of ischemic reperfusion. a–d Neurological deficits were evaluated at 24, 48, and 72 h after reperfusion. ZSTK474 markedly alleviate the modified neurological severity scores, adhesive removal test, and foot fault test compared to mice in the control group. There was no obvious change in corner test outcomes between ZSTK474-treated and control groups. e Representative TTC-stained brain sections of mice treated with 200 mg/kg ZSTK474 after MCAO (samples acquired at 72 h after stroke). f Quantitative analysis of the percentage of brain infarct volume. ZSTK474 treatment reduced the percentage of brain infarct volume to a level of statistically significant difference compared to the control group. g H&E staining showed that neurons had normal morphologic features in the sham-operated group. The MCAO group presented with multiple vacuolated interspaces, infiltration of inflammatory cells, and dead neurons. ZSTK474 significantly ameliorated the damage caused by reperfusion injury. h Quantitative analysis of the number of inflammatory cells in G. Data expressed as means ± SD; ^# P < 0.01, *P < 0.05, vs. control group; n = 6–10 per group