Fig. 3

Double immunofluorescent staining for the microglial/macrophage marker Iba-1 and detrimental or restorative state markers. Brain sections were prepared 72 h after MCAO. a Double immunofluorescent staining for CD16/32 (red; detrimental state marker) and Iba-1 (green). b Double immunofluorescent staining for CD206 (red; restorative state marker) and Iba-1 (green). c Quantification of the percentage of CD16/32⁺/Iba-1⁺ cells. d Quantification of the percentage of CD206⁺/Iba-1⁺ cell. e, f PCR was performed to quantify the mRNA expression of detrimental state markers (CD16, CD32, CD86) and restorative state markers (CD206, YM-1, Arg1) in the ipsilateral cortex 72 h after MCAO. Data in graphs are expressed as means ± SD; ^# P < 0.001, *P < 0.05 vs. control group; n = 3–6