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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Metabolic determinants of the immune modulatory function of neural stem cells

Fig. 1

Cytokine-primed NSCs hinder lymph node cell (LNC) proliferation and cause premature CD44 downregulation. Unfractionated LNC were labelled with the vital dye CFSE, cultured on CD3/CD28 coated mAb in the absence or the presence of NSCs, Th1 NSCs, or Th2 NSCs for 48 and 72 h in vitro. a Cells were surface stained with anti-CD4 and CD44 antibodies. Dot plots depict relative CD44 and CFSE levels and are representative of gated CD4+ viable cells. b–d Absolute fractions of CFSEdim (b), CFSEdimCD44low (c), and CFSEdimCD44high CD4+ T cells are shown (d). Data in b–d are expressed as mean % (±SD) and have been collected out of n ≥ 3 independent experiments. e Representative dot plots of relative CFSE and TO-PRO3 levels after gating on CD4+ T cells as in a–d. Unpaired two-tailed t test for comparisons between groups were applied; *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001 vs. LNC

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