Fig. 2
Social defeat does not increase macrophage cell trafficking to the brain. a Cell characterization strategy. Cells were first gated by DAPI exclusion and size to identify live cells, then gated to exclude doublets. Live single cells were then assessed for CD11b and CD45 staining. Representative bivariate dot plots show brain cells from HC, acute SD, or chronic SD mice stained and gated for microglia (CD11bhi CD45low) and macrophages (CD11bhi CD45hi). b Number of macrophages in the brain was similar between all treatment conditions. Results are expressed as percentage CD45hi cells in the CD11b hi cell population (n = 8 per group). One-way ANOVA detected no significance between groups