Fig. 3

Minimal effects of AT-RvD1 on spinal WDR neurones in MIA-treated rats. On day 28 post model induction (intra-articular 1 mg MIA or saline), AT-RvD1 15 and 150 ng/50 μl applied spinally did not alter spinal WDR neurone firing in saline-treated rats (a-f white bars). In MIA-treated rats (a-f grey bars), even at the higher concentration, AT-RvD1 significantly inhibited only Aδ responses (b). However, WDR neurone firings in both groups were markedly suppressed following 1 μg/50 μl morphine (Mor) application. Data expressed as mean maximal number of action potentials post drug application. *p < 0.05, **p < 0.01, ***p < 0.0001 repeated measure ANOVA with Sidak post hoc test. ^# p < 0.05 Friedman test with Dunn’s post hoc test. Saline n = 7, MIA n = 9. Note that two neurones from each group were excluded due to incomplete data sets (not receiving morphine) and identified as outliers