Fig. 4

BDNF and CB1R signaling in EAE striatum. a BDNF mRNA in EAE-vehicle striatum is upregulated with respect to control CFA-vehicle (p < 0.05). Such alteration is not corrected by amphetamine treatment. b shows WB of striatal lysates of CFA-vehicle, EAE-vehicle, and EAE-amphetamine. CB1R levels in the striatum are not affected by EAE, as indicated by WB analysis of protein lysates (b’). Amphetamine given 24 h before the sacrifice of the animals did not affect the total amount of CB1R in the EAE striatal lysates. CB1R signal was normalized to β-actin bands. WB data are normalized to CFA values. c, Representative WB of lysates from CFA-vehicle, EAE-vehicle, and amphetamine-vehicle probed with antibody specific for the carboxy-terminal phosphorylation of CB1R (p-Ser316): the histogram in c’ indicates a remarkable reduction of CB1R phosphorylation, normalized to β-actin, which is not modulated by amphetamine treatment. Values are means ± SEM. Statistical differences were analyzed by one-way ANOVA followed by Tukey HSD (WB and qPCR). *p < 0.05 EAE-vehicle vs CFA-vehicle