Fig. 4

CD11b and C3 confocal co-localization in WT, MASP-2^−/−, and MASP-1/3^−/− mice. Representative immunofluorescence micrographs of CD11b (red) and C3 deposition (green) in WT (a), MASP-2^−/− (b), and MASP-1/3^−/− (c) mice in the ischemic cortical lesion following 60 min of ischemia and 48 h of reperfusion indicating C3 reduced deposition in MASP-2^−/− mice (scale bar = 20 μm). Closer overview of CD11b-positive cells with the typical features engulfing C3-positive cells in WT (d) and MASP-1/3^−/− (f) and a less reactive CD11b-positive cell in close proximity to C3 in a MASP-2^−/− mouse (e). Nuclei are in blue (scale bar = 10 μm)