Fig. 7

miR-17-92 cluster overexpression in grafted NSCs reduces astrogliosis and improves the motor coordination of brain-injured mice. a Immunostaining for GFAP (red), 12 weeks after transplantation of CON-NSC or miR-17-92-NSC (green). The area within white dotted lines corresponds to the astroglial scar. Scale bar, 200 μm. b Quantification of astrogliosis, as measured by relative GFAP fluorescence intensity from all coronal sections containing the injury site along the rostro-caudal axis (*P < 0.05, unpaired two-tailed t test, n = 3 independent experiments). c The mice that received control NSC (CON-NSC), miR-17-92-overexpressing NSC (miR-17-92-NSC), or no cells (Only Injury) were tested 1, 4, 6, and 12 weeks after injury and transplantation. Asterisks indicate a statistically significant difference compared with the only injury group or CON-NSC group (*P < 0.05, one-way ANOVA followed by Tukey’s test, n = 8 mice)