Fig. 2

CNS-directed delivery of siponimod reduces astrogliosis in the EAE striatum. a Confocal microscopy images depict the striatum of EAE-vehicle and siponimod mice stained for the astrocyte marker GFAP (green fluorescence, counterstained with DAPI, gray). Expression of GFAP is markedly reduced in EAE-siponimod striatum compared to EAE-vehicle striatum (scale bar: 100 μm). The images are representative of stained sections from three animals per groups from two immunizations. The panel in b shows western blot comparing EAE-siponimod and EAE-vehicle striatal extracts probed for anti-GFAP antibody. Densitometric analysis of the bands in b’ reveals reduced GFAP content, relative to β-actin, in EAE-siponimod lysates compared to EAE-vehicle. WB data are normalized to EAE-vehicle values. Unpaired T test was used for two-group analysis. Values are means ± SEM, *p < 0.05