Fig. 8

Deletion of Erk2 leads to lessened gliosis during cuprizone feeding. Histological samples of corpus callosum were obtained from either Erk2 cKO or Erk2 ^flox/flox mice before and 1, 2, 3, and 4 weeks after the start of cuprizone feeding. In Erk2 ^flox/flox mice, immunoreactivity for GFAP and Iba-1 is increased at 4 weeks compared to untreated (a, c left), while such changes are less prominent in Erk2 cKO mice (a, c right). b, d During 4 weeks of cuprizone feeding, the immunoreactivity for GFAP (b) and Iba-1 (d) in Erk2 ^flox/flox gradually increase, while the trend is less prominent in Erk2 cKO (b, d: n = 3 for each time point; Student’s t test; *p < 0.05; **p < 0.01). Scale bars: a, c 100 μm