Fig. 6

Ex vivo fluorescence and second-harmonic generation imaging showing SHG of collagen (blue color), fluorescence of GFP in endothelial cells (green), and PKH26 fluorescence marker in red blood cells (red) in the brains of saline (a) and triple-dose (3 mg/kg at 0, 6, and 24 h) LPS-treated Tie2-GFP mice sacrificed at 2 days (b). All images are 3D reconstructions in enface (or true focus) geometry. Asterisk sign indicates RBC confined within the brain vasculature in a saline mouse (a), and arrows indicate extravasation of RBC from the brain vasculature into the brain parenchyma in a LPS-treated mouse (b). Superior perspective of 3D surface renderings of brain showing distribution of cerebral microbleeds (in red) in mice from the 7-day experiment treated with a triple-dosing regimen of LPS (1 mg/kg at 0, 6, and 24 h) (c) using T2*-weighted 3D FLASH MRI