Fig. 4
From: α-Synuclein deficiency promotes neuroinflammation by increasing Th1 cell-mediated immune responses
Increased proliferation of α-synuclein-deficient splenocytes associated with increased splenic IL-2 mRNA expression in aSyn−/− mice prior to onset of EAE. a Proliferation of CD4+ cells was measured by flow cytometry in splenocyte cultures after ex vivo recall with MOG35–55 (splenocytes harvested on day 10 post immunization in aSyn−/− and aSyn+/+ mice), revealing significantly increased proliferation of aSyn−/−-derived splenocytes (n = 4; *p < 0.05). b RT-PCR was performed on cDNA derived from total RNA that was isolated from the spleen of aSyn−/− and aSyn+/+ mice 10 days after induction of EAE. IL-2 mRNA expression was significantly increased in the spleen of aSyn−/− mice (n = 4; *p < 0.05)