Fig. 3

Inhibitory effects of bullatine A administered subcutaneously, in a single dose (3 mg/kg) or the cumulative dose range of 0.3 to 30 mg/kg, on mechanical allodynia and heat hyperalgesia in complete Freud’s adjuvant (CFA) induced inflammatory pain (a–e) and bone cancer pain (f–h) rats. The inflammatory model was induced by tibiotarsal joint injection of CFA (100 μL), and the bone cancer pain model was induced by tibia implantation of Walker 256 carcinoma cells. e, h Dose–response analysis of bullatine A on % maximum possible effect (% MPE) at 1 h after injection was best projected by the non-linear least-squares method. The data are presented as means ± SEM (n = 6 in each group). Symbol a denotes statistical significance compared with the saline control group (P < 0.05, two-way ANOVA followed by the post hoc Student–Newman–Keuls tests)